Publications

Pérez-Cruz, C.#, Moraleda-Montoya, A.#, Liébana, R.#, Terrones, O., Arrizabalaga, U., García-Alija, M., Lorizate, M., Martínez Gascueña, A., García-Álvarez, I., Ander Nieto-Garai, J., Olazar-Intxausti, O., Rodríguez-Colinas, B., Mann, E., Chiara, J.L., Contreras, F.-X.*, Guerin, M.E.*, Trastoy, B.*, Alonso-Sáez, S.* (2024) Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota. Nat. Comm. 15, 10906

Sastre, D.E., Sultana, N., Navarro, M.VAS., Huliciak, M-, Du J.J., Cifuente, J.O., Flowers, M., Liu, X., Lollar, P., Trastoy, B., Guerin, M.E., Sundberg, E.J. (2024). Human gut microbes express functionally distinct endoglycosidases to metabolize the same N-glycan substrate. Nat. Comm. 15, 5123

García-Alija, M., van Moer, B., Sastre, D.E., Azzam, T., Du, J.J., Trastoy, B.*, Callewaert, N., Sundberg, E.J.*, Guerin, M.E.* (2023). Modulating antibody effector functions by Fc glycoengineering. Biotechnol. Adv. 67, 108201

Du, J.J., Sastre, D.E., Trastoy, B., Roberts, B., Deredge, D., Klontz, E.H., Flowers, M.W., Sultana, N., Guerin, M.E., Sundberg, E.J. (2023) Mass Spectrometry-Based Methods to Determine the Substrate Specificities and Kinetics of N-Linked Glycan Hydrolysis by Endo-β-N-Acetylglucosaminidases. Bacterial Pathogenesis: Methods and Protocols. 147-167. Book chapter

Anso, I., Naegeli, A., Cifuente, J.O., Orrantia, A., Andersson, E., Zenarruzabeitia, O., Moraleda-Montoya, A., García-Alija, M., Corzana, F., Del Orbe, R.A., Borrego, F., Trastoy, B.*, Sjögren, J.*, Guerin, M.E.* (2023) Turning universal O into rare Bombay type blood. Nat. Comm. 14, 1765.

Trastoy, B.*, Du, J.J., Cifuente, J.O., Rudolph, L., García-Alija, M., Klontz, E.H., Deredge, D., Sultana, N., Huynh, C.G., Flowers, M.W., Li, C., Sastre, D.E., Wang, L.-X., Corzana, F., Mallagaray, A.*, Sundberg, E.J.*, Guerin, M.E.* (2023) Mechanism of antibody-specific deglycosylation and immune evasion by Streptococcal IgG-specific endoglycosidases. Nat. Comm. 14, 1705. Highlighted as Faculty Opinions article (2023).

Cioetto-Mazzabò, L., Boldrin, F., Beauvineau, C., Speth, M., Marina, A., Namouchi, A., Segafreddo, G., Cimino, M., Favre-Rochex, S., Balasingham, S., Trastoy, B., Munier-Lehmann, H., Griffiths, G., Gicquel, B., Guerin, M.E., Manganelli, R., Alonso-Rodríguez, N. (2023) SigH stress response mediates killing of Mycobacterium tuberculosis by activating nitronaphthofuran prodrugs via induction of Mrx2 expression. Nucleic Acids Res. 51, 144-165

García-Alija, M., Du, J. J., Ordoñez, I., Diz-Vallenilla, A., Moraleda-Montoya, A., Sultana, N., Huynh, C. G., Li, C., Donahue, T. C., Li, C., Wang, L.-X., Trastoy, B.*, Sundberg, E. J.*, Guerin, M. E.* (2022) Mechanism of cooperative N-glycan processing by the multi-modular endoglycosidase EndoE. Nat. Comm.  13, 1137. Nature Communications Editors’ Highlights (2022)

Trastoy, B.*, Du, J. J., García-Alija, M., Li, C., Klontz, E. H., Wang, L.-X., Sundberg, E. J.*, Guerin, M. E.* (2022) Sculpting therapeutic monoclonal antibody N-glycans using endoglycosidases. Curr. Opin. Struc. Biol. 72, 248-259 Review.

Anso, I., Basso, L. G. M., Wang, L., Marina, A., Páez-Pérez, E. D., Jäger, C., Gavotto, F., Tersa, M., Perrone, S., Contreras, F.-X., Prandi, J., Gilleron, M., Linster, C. L., Corzana, F., Lowary, T. L., Trastoy, B.*, and Guerin, M. E.* (2021) Molecular ruler mechanism and interfacial catalysis of the integral membrane acyltransferase PatA. Sci. Adv. 7, eabj4565.

Trastoy, B.*, Du, J. J., Li, C., García-Alija, M., Klontz, E.H., Roberts, B. R., Donahue, T. C., Wang, L.-X., Sundberg, E. J., Guerin, M. E. (2021) GH18 endo-β-N-acetylglucosaminidases use distinct mechanisms to process hybrid-type N-linked glycans. J. Biol. Chem. 297, 101011.

Trastoy, B., Naegeli, A., Anso, I., Sjögren, J., Guerin, M. E. (2020) Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila. Nat. Commun. 11, 4844.

Rodrigo-Unzueta, A., Ghirardello, M., Urresti, S., Delso, I., Gigante, D., Anso, I., Trastoy, B., Comino, N., Tersa, M., D’Angelo, C., Ciguente, J. O., Marina, A., Liebau, J., Mäler, L., Chenal, A., Albesa-Jové, D., Merino, P. and Guerin, M.E. (2020) Dissecting the Structural and Chemical Determinants of the “Open-to-Closed” Motion in the Mannosyltransferase PimA from Mycobacteria. Biochemistry. 59, 32, 2934-2945.

Liebau, J., Tersa, M., Trastoy, B., Patrick, J., Rodrigo-Unzueta, A., Corzana, F. Sparrman, T. Guerin, M. E., Mäler, L. (2020) Unveiling the activation dynamics of a fold-switch bacterial glycosyltransferase by ¹⁹F NMR. J. Bio. Chem. 295 (29) 9868-9878.

Trastoy, B.,* Du, J. J., Klontz, E. H., Li, C., Cifuente, J. O., Wang, L.X., Sundberg, E. J.,* Guerin, M. E.* (2020) Structural basis of mammalian high-mannose N-glycan processing by human gut Bacteroides. Nat. Commun. 11, 899. Highlighted as F1000Prime article (2020).

Sastre, D. E., Basso, L. G. M., Trastoy, B., Cifuente, J. O., Contreras, X., Gueiros-Filho, F., de Mendoza, D., Navarro, M. V. A. S., and Guerin, M. E. (2020) Membrane fluidity adjusts the insertion of the transacylase PlsX to regulate phospholipid biosynthesis in Gram-positive bacteria. J. Biol. Chem. 7, 2136-2147. Highlighted in the Lipid News section of ASBMB Today (2020).

Du, J. J., Klontz, E. H., Guerin, M. E., Trastoy, B.,* and Sundberg, E. J.* (2020) Structural insights into the mechanisms and specificities of IgG-active endoglycosidases. Glycobiology. 30, 268-279. Review

Klontz, E. H., Trastoy, B., Deredge, D., Fields, J. K., Li, C., Orwenyo, J., Marina, A., Beadenkopf, R., Günther, S., Flores, J., Wintrode, P. L., Wang, L. X., Guerin, M. E., and Sundberg, E. J. (2019) Molecular basis of broad spectrum N-glycan specificity and processing of therapeutic IgG monoclonal antibodies by Endoglycosidase S2. ACS Cent. Sci. 5, 524–538. Highlighted as F1000Prime article (2019).

Cifuente, J. O., Comino, N., Trastoy, B., D’Angelo, C., and Guerin, M. E. (2019) Structural basis of glycogen metabolism in bacteria. Biochem. J. 476(14) 2059-2092. Review.

Albesa-Jové, D., Cifuente, J. O., Trastoy, B., and Guerin M. E. (2019) Quick-soaking of crystals reveals unprecedented insights into the catalytic mechanism of glycosyltransferases. Methods Enzymol. 621, 261-279. Review

Trastoy, B., Klontz, E. H., Orwenyo, J., Marina, A., Wang, L.-X., Sundberg, E. J., and Guerin, M. E. (2018) Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S. Nat. Commun. 9, 1874.

Tersa, M., Raich, L., Albesa-Jové, D., Trastoy, B., Prandi, J., Gilleron, M., Rovira, C., and Guerin, M. E. (2018) The Molecular Mechanism of Substrate Recognition and Catalysis of the Membrane Acyltransferase PatA from Mycobacteria. ACS Chem. Biol. 13, 131–140. Editor’s choice.

Trastoy, B., Lomino, J. V., Pierce, B. G., Carter, L. G., Gunther, S., Giddens, J. P., Snyder, G. A., Weiss, T. M., Weng, Z., Wang, L.-X., and Sundberg, E. J. (2014) Crystal structure of Streptococcus pyogenes EndoS, an immunomodulatory endoglycosidase specific for human IgG antibodies. Proc. Natl. Acad. Sci. USA 111, 6714–6719.

Trastoy, B., Lomino, J. V., Wang, L.-X., and Sundberg, E. J. (2013) Liquid-liquid diffusion crystallization improves the X-ray diffraction of EndoS, an endo-β-N-acetylglucosaminidase from Streptococcus pyogenes with activity on human IgG. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 69 (12):1405-10.

Perez-Ojeda, M. E., Trastoy, B., Rol, A. Chiara, M. D., Garcia-Moreno, I. and Chiara, J. L. (2013) Controlled Click-Assembly of Well-Defined Hetero-Bifunctional Cubic Silsesquioxanes and Their Application in Targeted Bioimaging. Chem. Eur. J. 19(21) 6630-6640.

Trastoy, B., Bonsor, D. A., Pérez-Ojeda, M. E., Jimeno, M. L., Méndez-Ardoy, A., García Fernández, J. M., Sundberg, E. J., and Chiara, J. L. (2012) Synthesis and biophysical study of disassembling nanohybrid bioconjugates with a cubic octasilsesquioxane core. Adv. Funct. Mater. 22(15), 3191–3201.

Perez-Ojeda, M.E., Trastoy, B., Lopez-Arbeloa, I., Banuelos, J.,  Costela, A., Garcia-Moreno, I., and Chiara, J. L. (2011) Click Assembly of Dye-Functionalized Octasilsesquioxanes for Highly Efficient and Photostable Photonic Systems. Chem. Eur. J. 17(47) 13258-13268.

Suarez, J. R., Trastoy, B., Perez-Ojeda, M. E., Marin-Barrios, R. and Chiara, J. L. (2010) Nonafluorobutanesulfonyl Azide: A Shelf-Stable Diazo Transfer Reagent for the Synthesis of Azides from Primary Amines. Adv. Synth. Catal. 14(15) 2515-2520.

Trastoy, B., Pérez-Ojeda, M. E., Sastre, R., and Chiara, J. L. (2010) Octakis(3-azidopropyl)octasilsesquioxane: A versatile nanobuilding block for the efficient preparation of highly functionalized cube-octameric polyhedral oligosilsesquioxane frameworks through click assembly. Chem. Eur. J. 16, 3833–3841.

Sastre, R., Martin, V., Garrido, L., Chiara, J.L., Trastoy, B., Garcia, O., Costela, A., and Garcia-Moreno, I. (2009) Dye-Doped Polyhedral Oligomeric Silsesquioxane (POSS)-Modified Polymeric Matrices for Highly Efficient and Photostable Solid-State Lasers. Adv. Funct. Mater. 19(20) 3307-3316.